A simple and general route for monofunctionalization of fluorescent and magnetic nanoparticles using peptides.
Identifieur interne : 001619 ( Main/Exploration ); précédent : 001618; suivant : 001620A simple and general route for monofunctionalization of fluorescent and magnetic nanoparticles using peptides.
Auteurs : RBID : pubmed:21411925English descriptors
- KwdEn :
- Cadmium Compounds (chemistry), Fluorescent Dyes (analysis), Fluorescent Dyes (chemistry), HeLa Cells, Humans, Indium (chemistry), Magnetics, Microscopy, Electron, Transmission, Nanoparticles (chemistry), Nanoparticles (ultrastructure), Nanotechnology (methods), Particle Size, Peptides (chemistry), Phosphines (chemistry), Quantum Dots, Selenium Compounds (chemistry), Sulfides (chemistry), Surface Properties, Zinc Compounds (chemistry).
- MESH :
- chemical , analysis : Fluorescent Dyes.
- chemical , chemistry : Cadmium Compounds, Fluorescent Dyes, Indium, Peptides, Phosphines, Selenium Compounds, Sulfides, Zinc Compounds.
- chemistry : Nanoparticles.
- methods : Nanotechnology.
- ultrastructure : Nanoparticles.
- HeLa Cells, Humans, Magnetics, Microscopy, Electron, Transmission, Particle Size, Quantum Dots, Surface Properties.
Abstract
Nanoparticles are now utilized in many diverse biological and medical applications. Despite this, it remains challenging to tailor their surface for specific molecular targeting while maintaining high biocompatibility. To address this problem, we evaluate a phytochelatin-related peptide surface coating to produce functional and biocompatible nanoparticles (NPs) based on fluorescent InP/ZnS and CdSe/ZnS or superparamagnetic FePt and Fe(3)O(4). Using a combination of transmission electron microscopy, size-exclusion chromatography and gel electrophoresis (GE), we demonstrate the excellent colloidal properties of the peptide-coated NPs (pNPs) and the compact nature of the coating (∼4 nm thickness). We develop a simple protocol for the monofunctionalization of the pNPs with targeting biomolecules, by combining covalent conjugation with GE purification. We then employ functionalized InP/ZnS pNPs in a live-cell, single-molecule imaging application to specifically target and detect individual proteins in the cell membrane. These findings showcase the versatility of the peptides for preparing compact NPs of various compositions and sizes, which are easily functionalized, and suitable for a broad range of biomedical applications.
DOI: 10.1088/0957-4484/22/17/175103
PubMed: 21411925
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A simple and general route for monofunctionalization of fluorescent and magnetic nanoparticles using peptides.</title><author><name sortKey="Clarke, Samuel" uniqKey="Clarke S">Samuel Clarke</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire Kastler Brossel, CNRS UMR 8552, Département de Physique et Institut de Biologie, Ecole Normale Supérieure, Université Pierre et Marie Curie (Paris6), 46 rue d'Ulm 75005 Paris, France. samuel.clarke@lkb.ens.fr</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire Kastler Brossel, CNRS UMR 8552, Département de Physique et Institut de Biologie, Ecole Normale Supérieure, Université Pierre et Marie Curie (Paris6), 46 rue d'Ulm 75005 Paris</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Tamang, Sudarsan" uniqKey="Tamang S">Sudarsan Tamang</name></author><author><name sortKey="Reiss, Peter" uniqKey="Reiss P">Peter Reiss</name></author><author><name sortKey="Dahan, Maxime" uniqKey="Dahan M">Maxime Dahan</name></author></titleStmt><publicationStmt><date when="2011">2011</date><idno type="doi">10.1088/0957-4484/22/17/175103</idno><idno type="RBID">pubmed:21411925</idno><idno type="pmid">21411925</idno><idno type="wicri:Area/Main/Corpus">001505</idno><idno type="wicri:Area/Main/Curation">001505</idno><idno type="wicri:Area/Main/Exploration">001619</idno></publicationStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cadmium Compounds (chemistry)</term><term>Fluorescent Dyes (analysis)</term><term>Fluorescent Dyes (chemistry)</term><term>HeLa Cells</term><term>Humans</term><term>Indium (chemistry)</term><term>Magnetics</term><term>Microscopy, Electron, Transmission</term><term>Nanoparticles (chemistry)</term><term>Nanoparticles (ultrastructure)</term><term>Nanotechnology (methods)</term><term>Particle Size</term><term>Peptides (chemistry)</term><term>Phosphines (chemistry)</term><term>Quantum Dots</term><term>Selenium Compounds (chemistry)</term><term>Sulfides (chemistry)</term><term>Surface Properties</term><term>Zinc Compounds (chemistry)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Fluorescent Dyes</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Cadmium Compounds</term><term>Fluorescent Dyes</term><term>Indium</term><term>Peptides</term><term>Phosphines</term><term>Selenium Compounds</term><term>Sulfides</term><term>Zinc Compounds</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Nanoparticles</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Nanotechnology</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Nanoparticles</term></keywords><keywords scheme="MESH" xml:lang="en"><term>HeLa Cells</term><term>Humans</term><term>Magnetics</term><term>Microscopy, Electron, Transmission</term><term>Particle Size</term><term>Quantum Dots</term><term>Surface Properties</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Nanoparticles are now utilized in many diverse biological and medical applications. Despite this, it remains challenging to tailor their surface for specific molecular targeting while maintaining high biocompatibility. To address this problem, we evaluate a phytochelatin-related peptide surface coating to produce functional and biocompatible nanoparticles (NPs) based on fluorescent InP/ZnS and CdSe/ZnS or superparamagnetic FePt and Fe(3)O(4). Using a combination of transmission electron microscopy, size-exclusion chromatography and gel electrophoresis (GE), we demonstrate the excellent colloidal properties of the peptide-coated NPs (pNPs) and the compact nature of the coating (∼4 nm thickness). We develop a simple protocol for the monofunctionalization of the pNPs with targeting biomolecules, by combining covalent conjugation with GE purification. We then employ functionalized InP/ZnS pNPs in a live-cell, single-molecule imaging application to specifically target and detect individual proteins in the cell membrane. These findings showcase the versatility of the peptides for preparing compact NPs of various compositions and sizes, which are easily functionalized, and suitable for a broad range of biomedical applications.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">21411925</PMID><DateCreated><Year>2011</Year><Month>03</Month><Day>17</Day></DateCreated><DateCompleted><Year>2011</Year><Month>07</Month><Day>18</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1361-6528</ISSN><JournalIssue CitedMedium="Internet"><Volume>22</Volume><Issue>17</Issue><PubDate><Year>2011</Year><Month>Apr</Month><Day>29</Day></PubDate></JournalIssue><Title>Nanotechnology</Title><ISOAbbreviation>Nanotechnology</ISOAbbreviation></Journal><ArticleTitle>A simple and general route for monofunctionalization of fluorescent and magnetic nanoparticles using peptides.</ArticleTitle><Pagination><MedlinePgn>175103</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1088/0957-4484/22/17/175103</ELocationID><Abstract><AbstractText>Nanoparticles are now utilized in many diverse biological and medical applications. Despite this, it remains challenging to tailor their surface for specific molecular targeting while maintaining high biocompatibility. To address this problem, we evaluate a phytochelatin-related peptide surface coating to produce functional and biocompatible nanoparticles (NPs) based on fluorescent InP/ZnS and CdSe/ZnS or superparamagnetic FePt and Fe(3)O(4). Using a combination of transmission electron microscopy, size-exclusion chromatography and gel electrophoresis (GE), we demonstrate the excellent colloidal properties of the peptide-coated NPs (pNPs) and the compact nature of the coating (∼4 nm thickness). We develop a simple protocol for the monofunctionalization of the pNPs with targeting biomolecules, by combining covalent conjugation with GE purification. We then employ functionalized InP/ZnS pNPs in a live-cell, single-molecule imaging application to specifically target and detect individual proteins in the cell membrane. These findings showcase the versatility of the peptides for preparing compact NPs of various compositions and sizes, which are easily functionalized, and suitable for a broad range of biomedical applications.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Clarke</LastName><ForeName>Samuel</ForeName><Initials>S</Initials><Affiliation>Laboratoire Kastler Brossel, CNRS UMR 8552, Département de Physique et Institut de Biologie, Ecole Normale Supérieure, Université Pierre et Marie Curie (Paris6), 46 rue d'Ulm 75005 Paris, France. samuel.clarke@lkb.ens.fr</Affiliation></Author><Author ValidYN="Y"><LastName>Tamang</LastName><ForeName>Sudarsan</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Reiss</LastName><ForeName>Peter</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Dahan</LastName><ForeName>Maxime</ForeName><Initials>M</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType>Journal Article</PublicationType><PublicationType>Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2011</Year><Month>03</Month><Day>16</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Nanotechnology</MedlineTA><NlmUniqueID>101241272</NlmUniqueID><ISSNLinking>0957-4484</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Cadmium Compounds</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Fluorescent Dyes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Peptides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Phosphines</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Selenium Compounds</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Sulfides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Zinc Compounds</NameOfSubstance></Chemical><Chemical><RegistryNumber>045A6V3VFX</RegistryNumber><NameOfSubstance>Indium</NameOfSubstance></Chemical><Chemical><RegistryNumber>22398-80-7</RegistryNumber><NameOfSubstance>indium phosphide</NameOfSubstance></Chemical><Chemical><RegistryNumber>A7F646JC5C</RegistryNumber><NameOfSubstance>cadmium selenide</NameOfSubstance></Chemical><Chemical><RegistryNumber>KPS085631O</RegistryNumber><NameOfSubstance>zinc sulfide</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N">Cadmium Compounds</DescriptorName><QualifierName MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Fluorescent Dyes</DescriptorName><QualifierName MajorTopicYN="N">analysis</QualifierName><QualifierName MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">HeLa Cells</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Indium</DescriptorName><QualifierName MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Magnetics</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Microscopy, Electron, Transmission</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Nanoparticles</DescriptorName><QualifierName MajorTopicYN="Y">chemistry</QualifierName><QualifierName MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Nanotechnology</DescriptorName><QualifierName MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Particle Size</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Peptides</DescriptorName><QualifierName MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Phosphines</DescriptorName><QualifierName MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Quantum Dots</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Selenium Compounds</DescriptorName><QualifierName MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Sulfides</DescriptorName><QualifierName MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Surface Properties</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Zinc Compounds</DescriptorName><QualifierName MajorTopicYN="N">chemistry</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="aheadofprint"><Year>2011</Year><Month>3</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2011</Year><Month>3</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2011</Year><Month>3</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2011</Year><Month>7</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pii">S0957-4484(11)78166-1</ArticleId><ArticleId IdType="doi">10.1088/0957-4484/22/17/175103</ArticleId><ArticleId IdType="pubmed">21411925</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=IndiumV2/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001619 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001619 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= *** parameter Area/wikiCode missing ***
|area= IndiumV2
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:21411925
|texte= A simple and general route for monofunctionalization of fluorescent and magnetic nanoparticles using peptides.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:21411925" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a IndiumV2
| This area was generated with Dilib version V0.5.76. |  |